These characters are Thomas and Twilight Sparkle's enemies.
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A
- A.I.M.
- Aaravos
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- Abacus Cinch
- Abbadomon
- Abigail Williams
- Abis Mal
- Abis Mal's Men
- Abomination
- Abominus
- Abrakadanger
- Acat
- Accelerator
- Ace Gecko
- Acid Storm
- Acnologia
- Acronix
- Adam Taurus
- Admiral Malkor
- Adonis
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- Adrena Lynn
- Adrien's bodyguard
- Afterburner (Cyberverse)
- Agatha Harkness
- Agatha Trunchbull
- Agent Kalaschikov
- Agent Kallus
- Agent Kent B. Trusted
- Agent P. Cleary
- Agent Trout
- Agent Woods
- Aggie Prenderghast
- Aggregor
- Agnese Sanctis
- Ahuizotl
- Ai and Mako's Impmon
- Air Elementor
- Air Pirates
- Airachnid
- Airazor (Decepticon)
- Airdramon
- Aiwei
- Ajatarmon
- Ajeel Raml
- Akita
- Akito Sohma
- Aku
- Aku's Minions
- Akuma
- Akumatized villains
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- Al McWhiggin
- Al-G Rhythm
- Alador Blight
- Alameda Slim
- Alan and Ann
- Alaskan Bull Worm
- Albedo
- Albert Wesker
- Alchemist
- Alcyone
- Aldith
- Alejandro
- Alejandro Gutierrez
- Alexandra Springer
- Alfred the Loaned B12 engine
- Algard Von Palettia
- Ali and Bobo
- Aliana
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- Aliens (Mr. Men Show)
- Aliens (Xenomorphs)
- Alisa Silvermane
- Alister Azimuth
- All for One
- Alley Cat Gang
- Allomere
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- Alma Madrigal
- Almighty Tallest Red and Purple
- Aloysius O'Hare
- Alpha
- Alpha Nanite
- Alpha Strike
- Alpha-1
- Alphonse LaFleur
- Alphonse Perrier du von Scheck
- Alternate World Team Rocket
- Alva
- Alvin (Tales)
- Alvin the Treacherous
- Alyssa
- Amanda Banshee
- Amanda Evert
- Amanda Killman
- Amanda Waller (DC)
- Amazon Quartet
- Amber (Dawn of the Croods)
- Amelia Von Butch
- Amelie Graham de Vanily
- Amin Damoola
- Amity Blight
- Ammonia Pine
- Amond
- Amorpho
- Amos Slade
- Amuk Moonrah
- Amy and Samey
- Anakin Skywalker
- Anansi (Character)
- Anastasia Tremaine
- Ancestral Witches
- Ancient Minister
- AncientSphinxmon
- Anck-Su-Namun
- Andre
- Andrea Davenport
- Andre` LeBlanc
- Android 13
- Android 14
- Android 15
- Android 17
- Android 19
- Andromon
- Andross
- Angel (Teen Titans)
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- Angelene
- Angelica
- Angelica Pickles
- Angila
- Angry Archer
- Animatronic Captain Hook
- Animatronic Hieronymus Glove
- Animus (Dragon)
- Ankylosaurus (Ice Age)
- Ansem, Seeker of Darkness
- Anthony the Weasel
- Anti
- Anti-Cosmo
- Anti-Fairies
- Anti-Pops
- Anti-Recess Legion
- Anti-Sparky
- Anti-Wanda
- Antibug (Character)
- Anton Ego
- Apemon
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- Apocalymon
- Apocalypse
- Apollomon
- Appledoom
- Arcadiamon (Ultimate)
- Archie
- Ares
- Ares (God of War)
- Ares (Marvel)
- Aresia
- Argor Zardok
- Argus
- Aribb
- Arkady Duvall
- Arlong
- Armadylan
- Armaggon
- Armand D'Argencourt
- Armondo Guitierrez
- Armored Armadillo
- Army of Scar
- Arnim Zola
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- Arnold Royalton
- Arnold Stromwell
- Arnold the Pit Bull
- Arpeggio
- Arrby
- Arry and Bert
- Arthur and Cecil
- Arthur Slugworth
- Arthur Slugworth (Wonka)
- Arthur Watts
- Artorius Collbrande
- Arukenimon
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- Asajj Ventress
- Asch
- Ascot