2nd Dimension Charlene Doofenshmirtz is the main antagonist of the Phineas and Ferb episode "Tales From the Resistance: Back to the 2nd Dimension", the sequel to the 2011 TV film Phineas and Ferb The Movie: Across the 2nd Dimension. She is an alternate reality version of Charlene Doofenshmirtz (the ex-wife of Heinz Doofenshmirtz), but turns out to be very evil and intelligent as her husband 2nd Dimension Heinz Doofenshmirtz and their daughter 2nd Dimension Vanessa Doofenshmirtz.
Just like her original counterpart, she was voiced by Allison Janney, who previously voiced Ms. Grunion in Peabody and Sherman, Gladys Sharp in Over the Hedgeand Margaux Needler in The Addams Family.