Caroline, currently known as the Genetic Lifeform and Disk Operating System, or known as GLaDOS for an abbreviation, is the main antagonist of the first-person puzzle game franchise Portal, serving as the main antagonist of the first game and the deuteragonist of Portal 2 following Wheatley's betrayal. For the entirety of the first game and the first half of the second game, she is Chell's arch-nemesis.
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